Original investigation

Interaction between Calpain 5, Peroxisome proliferator-activated receptor-gamma and Peroxisome proliferator-activated receptor-delta genes: a polygenic approach to obesity

María E Sáez¹ , Antonio Grilo² , Francisco J Morón¹ , Luis Manzano³ , María T Martínez-Larrad⁵ , Antonio González-Pérez¹ , Javier Serrano-Hernando⁴ , Agustín Ruiz¹ , Reposo Ramírez-Lorca¹ and Manuel Serrano-Ríos⁵

¹  Departamento de Genómica Estructural, Neocodex, C/.,Charles Darwin 6, Acc. A, 41092, Sevilla, Spain

²  Departamento de Medicina Interna, Unidad de Hipertensión, Hospital Universitario de Valme, Ctra, Sevilla-Cádiz S/N, 41014, Sevilla, Spain

³  Departamento de Medicina Interna, Unidad de Insuficiencia Cardiaca y Riesgo Vascular, Hospital Ramón y Cajal, Crta, De Colmenar Viejo Km, 9100, 28034, Madrid, Spain

⁴  Departamento de Angiología Vascular, Hospital Clínico San Carlos, C/Profesor Martín Lagos s/n, 28040, Madrid, Spain

⁵  Departamento de Medicina Interna II, Hospital Clínico San Carlos, C/Profesor Martín Lagos s/n, 28040, Madrid, Spain

author email corresponding author email

Cardiovascular Diabetology 2008, 7:23doi:10.1186/1475-2840-7-23

Published:	25 July 2008

Abstract

Context

Obesity is a multifactorial disorder, that is, a disease determined by the combined effect of genes and environment. In this context, polygenic approaches are needed.

Objective

To investigate the possibility of the existence of a crosstalk between the CALPAIN 10 homologue CALPAIN 5 and nuclear receptors of the peroxisome proliferator-activated receptors family.

Design

Cross-sectional, genetic association study and gene-gene interaction analysis.

Subjects

The study sample comprise 1953 individuals, 725 obese (defined as body mass index ≥ 30) and 1228 non obese subjects.

Results

In the monogenic analysis, only the peroxisome proliferator-activated receptor delta (PPARD) gene was associated with obesity (OR = 1.43 [1.04–1.97], p = 0.027). In addition, we have found a significant interaction between CAPN5 and PPARD genes (p = 0.038) that reduces the risk for obesity in a 55%.

Conclusion

Our results suggest that CAPN5 and PPARD gene products may also interact in vivo.