Figure 5.

Palmitate induces translocation of cytochrome-c in cultured HMC. (A) Cells treated with control (Con, i.e., albumin alone), palmitate (Palm, 0.4 mM), oleate (Ole, 0.4 mM), or staurosporine (St, 1 μM) for the times indicated. HMC were then rapidly separated into soluble cytosolic and insoluble membrane fractions. The amount of cytochrome-c (Cyto-C) was determined by Western blotting. Equivalent amounts of total protein were present across all lanes of the cytoplasmic and membrane fractions, but the membrane fractions contained approximately 3 times more protein than the corresponding lane in the cytoplasmic fraction. (B) Densitometric analysis of cytoplasmic p14 kDa cytochrome-c from 3 independent experiments. Data are mean ± SEM. **, P < 0.01 by ANOVA versus control and oleate alone.