Figure 4.

Palmitate but not oleate increases caspase 9 enzyme activity. HMC were treated with palmitate (0.2 and 0.4 mM) or oleate (0.4 mM) for 24 and 48 h. Caspase 9 activity was then measured in HMC extracts adjusted for total protein using a luminometric assay with an LEHD peptide substrate as described in Materials and Methods. Data are mean ± SEM for n = 3 experiments in duplicate. **, P < 0.01, *, P < 0.05 versus control or oleate alone by ANOVA.