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Open Access Original investigation

Streptozotocin-induced diabetes prolongs twitch duration without affecting the energetics of isolated ventricular trabeculae

June-Chiew Han1*, Kenneth Tran1, Poul MF Nielsen12, Andrew J Taberner12 and Denis S Loiselle13

Author Affiliations

1 Auckland Bioengineering Institute, The University of Auckland, Auckland, New Zealand

2 Department of Engineering Science, The University of Auckland, Auckland, New Zealand

3 Department of Physiology, The University of Auckland, Auckland, New Zealand

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Cardiovascular Diabetology 2014, 13:79  doi:10.1186/1475-2840-13-79

Published: 15 April 2014

Abstract

Background

Diabetes induces numerous electrical, ionic and biochemical defects in the heart. A general feature of diabetic myocardium is its low rate of activity, commonly characterised by prolonged twitch duration. This diabetes-induced mechanical change, however, seems to have no effect on contractile performance (i.e., force production) at the tissue level. Hence, we hypothesise that diabetes has no effect on either myocardial work output or heat production and, consequently, the dependence of myocardial efficiency on afterload of diabetic tissue is the same as that of healthy tissue.

Methods

We used isolated left ventricular trabeculae (streptozotocin-induced diabetes versus control) as our experimental tissue preparations. We measured a number of indices of mechanical (stress production, twitch duration, extent of shortening, shortening velocity, shortening power, stiffness, and work output) and energetic (heat production, change of enthalpy, and efficiency) performance. We calculated efficiency as the ratio of work output to change of enthalpy (the sum of work and heat).

Results

Consistent with literature results, we showed that peak twitch stress of diabetic tissue was normal despite suffering prolonged duration. We report, for the first time, the effect of diabetes on mechanoenergetic performance. We found that the indices of performance listed above were unaffected by diabetes. Hence, since neither work output nor change of enthalpy was affected, the efficiency-afterload relation of diabetic tissue was unaffected, as hypothesised.

Conclusions

Diabetes prolongs twitch duration without having an effect on work output or heat production, and hence efficiency, of isolated ventricular trabeculae. Collectively, our results, arising from isolated trabeculae, reconcile the discrepancy between the mechanical performance of the whole heart and its tissues.

Keywords:
STZ-induced diabetes; Cardiac work; Cardiac heat production; Cardiac efficiency; Cardiac twitch duration