Role of MAP kinase in the regulation by insulin of D1 receptor expression and phosphorylation in A10 cells. A10 cells were treated with insulin (10-7M) in the presence or absence of the MAP kinase inhibitor PD98059 (10-6M) for 24 hrs. D1 receptor expression (A) or phosphorylation (B) was determined by immunoblotting or co-immunoprecipitation. D1 receptor serine phosphorylation was normalized by D1 receptor protein. (*P < 0.01, vs. control; #P < 0.05, vs. insulin, n = 5).
Fu et al. Cardiovascular Diabetology 2014 13:50 doi:10.1186/1475-2840-13-50