D1 receptor expression and phosphorylation in mesenteric arteries from lean and obese Zucker rats. (A): D1 receptor expression was determined by immunoblotting. (B): Serine-phosphorylated D1 receptor in rat mesenteric arteries was determined by co-immunoprecipitation and immunoblotting. Normal rabbit IgG (negative control) or D1 receptor antibody (positive control) was used as the immunoprecipitant. D1 receptor serine phosphorylation was normalized by D1 receptor protein. (*P < 0.05 vs. lean rats, n = 6).
Fu et al. Cardiovascular Diabetology 2014 13:50 doi:10.1186/1475-2840-13-50