Email updates

Keep up to date with the latest news and content from Cardiovascular Diabetology and BioMed Central.

Open Access Highly Accessed Original investigation

Regional evidence of modulation of cardiac adiponectin level in dilated cardiomyopathy: pilot study in a porcine animal model

Chiara Caselli1, Vincenzo Lionetti2, Manuela Cabiati1, Tommaso Prescimone1, Giovanni D Aquaro3, Virginia Ottaviano4, Fabio Bernini3, Letizia Mattii5, Silvia Del Ry1 and Daniela Giannessi1*

Author Affiliations

1 Consiglio Nazionale delle Ricerche (CNR), Institute of Clinical Physiology, Laboratory of Cardiovascular Biochemistry, Pisa, Italy

2 Department of Medicine, Scuola Superiore Sant’Anna, Pisa, Italy

3 Fondazione CNR-Regione Toscana “G. Monasterio”, Pisa, Italy

4 Department of Experimental Pathology BMIE, Faculty of Medicine, University of Pisa, Pisa, Italy

5 Human Section of Histology and Medical Embryology, Department of Human Morphology and Applied Biology, University of Pisa, Pisa, Italy

For all author emails, please log on.

Cardiovascular Diabetology 2012, 11:143  doi:10.1186/1475-2840-11-143

Published: 19 November 2012

Abstract

Background

The role of systemic and myocardial adiponectin (ADN) in dilated cardiomyopathy is still debated. We tested the regulation of both systemic and myocardial ADN and the relationship with AMP-activated protein kinase (AMPK) activity in a swine model of non-ischemic dilated cardiomyopathy.

Methods and results

Cardiac tissue was collected from seven instrumented adult male minipigs by pacing the left ventricular (LV) free wall (180 beats/min, 3 weeks), both from pacing (PS) and opposite sites (OS), and from five controls. Circulating ADN levels were inversely related to global and regional cardiac function. Myocardial ADN in PS was down-regulated compared to control (p < 0.05), yet ADN receptor 1 was significantly up-regulated (p < 0.05). No modifications of AMPK were observed in either region of the failing heart. Similarly, myocardial mRNA levels of PPARγ, PPARα, TNFα, iNOS were unchanged compared to controls.

Conclusions

Paradoxically, circulating ADN did not show any cardioprotective effect, confirming its role as negative prognostic biomarker of heart failure. Myocardial ADN was reduced in PS compared to control in an AMPK-independent fashion, suggesting the occurrence of novel mechanisms by which reduced cardiac ADN levels may regionally mediate the decline of cardiac function.

Keywords:
Adiponectin receptors; Heart failure; Animal models; AMPK; Adiponectin