Sympathetic nerve density assessment by tyrosine hydroxylase immunostaining of left ventricle sections at 8 weeks after STZ or vehicle administration. Representative slides from vehicle-treated control, STZ-treated euglycemic, and STZ-treated hyperglycemic rats (A). 20 × magnification; white arrowheads indicate tyrosine-hydroxylase positive nerve terminals. Relative sympathetic nerve density as percentage tyrosine-hydroxylase-positive area per field of view (B).
Thackeray et al. Cardiovascular Diabetology 2011 10:75 doi:10.1186/1475-2840-10-75